diff --git a/lib/std/Io/Threaded.zig b/lib/std/Io/Threaded.zig
index b9a0474de99359e7f6f04f50e5a222dc466a06c5..212ee2fba18bcff3c33bcdaf8ae66fb7dff8fd9c 100644
--- a/lib/std/Io/Threaded.zig
+++ b/lib/std/Io/Threaded.zig
@@ -7987,6 +7987,9 @@ fn fileReadStreamingWindows(userdata: ?*anyopaque, file: File, data: []const []u
             .LOCK_VIOLATION => return syscall.fail(error.LockViolation),
             .ACCESS_DENIED => return syscall.fail(error.AccessDenied),
             .INVALID_HANDLE => return syscall.fail(error.NotOpenForReading),
+            // TODO: Determine if INVALID_FUNCTION is possible in more scenarios than just passing
+            // a handle to a directory.
+            .INVALID_FUNCTION => return syscall.fail(error.IsDir),
             else => |err| {
                 syscall.finish();
                 return windows.unexpectedError(err);
@@ -8144,6 +8147,9 @@ fn fileReadPositionalWindows(userdata: ?*anyopaque, file: File, data: []const []
             .LOCK_VIOLATION => return syscall.fail(error.LockViolation),
             .ACCESS_DENIED => return syscall.fail(error.AccessDenied),
             .INVALID_HANDLE => return syscall.fail(error.NotOpenForReading),
+            // TODO: Determine if INVALID_FUNCTION is possible in more scenarios than just passing
+            // a handle to a directory.
+            .INVALID_FUNCTION => return syscall.fail(error.IsDir),
             else => |err| {
                 syscall.finish();
                 return windows.unexpectedError(err);
diff --git a/lib/std/fs/test.zig b/lib/std/fs/test.zig
index 63c51e6ed5b7429af7be18379a41fa30cd9c81d3..59cc05f1c1ec7faa893555a0c312f92a5fa6e8d0 100644
--- a/lib/std/fs/test.zig
+++ b/lib/std/fs/test.zig
@@ -847,7 +847,16 @@ test "file operations on directories" {
 
             {
                 const handle = try ctx.dir.openFile(io, test_dir_name, .{ .allow_directory = true, .mode = .read_only });
-                handle.close(io);
+                defer handle.close(io);
+
+                // Reading from the handle should fail
+                const expected_err = switch (native_os) {
+                    .wasi => error.NotOpenForReading,
+                    else => error.IsDir,
+                };
+                var buf: [1]u8 = undefined;
+                try expectError(expected_err, handle.readStreaming(io, &.{&buf}));
+                try expectError(expected_err, handle.readPositional(io, &.{&buf}, 0));
             }
             try expectError(error.IsDir, ctx.dir.openFile(io, test_dir_name, .{ .allow_directory = false, .mode = .read_only }));